Journal: Microchemical Journal

Article Title: Rapid determination of multiple aminoglycoside antibiotics in veterinary formulations by ion-pair chromatography coupled with evaporative light scattering detection

doi: 10.1016/j.microc.2021.106843

Figure Lengend Snippet: Fig. 2. Examples of chromatograms obtained using different types of LC columns, note that non-optimal composition of the mobile phase was used: MeOH with water both acidified with 0.1% HFBA. In the case of the Hypercarb column no peaks were registered even after 30 min of analysis. Peak identification (determined by analysis of standards of individual AGs): 1 – streptomycin, 2 – amikacin, 3 – kanamycin, 4 – paromomycin, 5 – apramycin, 6 – tobramycin, 7 – gentamicin (C1, C2, C1A), 8 – neomycin.

Article Snippet: Standards: amikacin sulphate (Interquim, CAS 39831–55-5, European Pharmacopoeia (EP) Reference Standard), apramycin sulphate (Alfa Aesar, CAS 70560–51-9, 96% purity), gentamicin sulphate as a sum of gentamicin C1, C2 and C1A (Merck, CAS 1405–41-0, ≥ 99% purity), kanamycin sulphate (Sigma-Aldrich, CAS 70560–51-9, ≥ 99.5% purity), neomycin sulphate (VWR, CAS 1405-10-3, Ultra-Pure Grade), paromomycin sulphate (Alfa Aesar, CAS 1263–89-4, ≥ 97% purity), streptomycin sulphate (Sigma-Aldrich, CAS 3810-74-0, ≥ 99% purity), tobramycin sulphate (Alfa Aesar, CAS 79645–27-5, ≥ 99% purity).

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